Hygromycin phosphotransferase hpt

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August undefined, 2024

Web(Empty Backbone) Constitutive CRISPRi vector conferring hygromycin B resistance, ... Aminoglycoside phosphotransferase. Use. CRISPR Tags. Expression. Mammalian ... Webaminoglycoside phosphotransferase. Hygromycin B (HygB, Wako Pure Chemical Industries, Osaka, Japan) was added to YPD to a ﬁnal concentration of 600mg/ml to …

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WebWe also show that a chimaeric gene consisting of the nopaline synthase (nos) gene regulatory elements and the E. coli derived hygromycin phosphotransferase (hpt) … Web1) hygromycin phosphotransferase (hpt) 潮霉素磷酸转移酶基因 (hpt) 2) hygB. 潮霉素磷酸转移酶基因. 1. We transformed the nematode-trapping fungus Arthrobotrys vermicola … indp ticker

Hygromycin Resistance Gene Cassettes for Vector Construction and

Web25 sep. 2015 · To date, however, only the hygromycin phosphotransferase gene (hpt) has been available for use as a marker for Agrobacterium-mediated transformation in M. … Web11 jun. 2024 · The hygromycin B phosphotransferase gene (hpt) has been widely used in the process of plant genetic engineering to produce plants that can secrete the … WebHygromycine phosphotransferase encoded by hpt gene is one of commonly used as a selectable marker for the production of transgenic plants. The plants harbored the hpt … loft rewards credit card

(Open Access) Transformation of the dermatophyte Trichophyton ...

Hygromycin phosphotransferase hpt

Development of Gateway Binary Vector Series with Four Different …

WebStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles. Target Full Name Hygromycin Phosphotransferase (HPT) Q & As … Webcultivated with Agrobacterium strain EHA101 containing a binary vector pTCL5, having hygromycin phosphotransferase (HPT) gene which confers resistance to hygromycin …

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WebRui Zhang is an academic researcher. The author has contributed to research in topic(s): Cotyledon & Transformation (genetics). The author has an hindex of 1, co-authored 1 … Web400052. Hygromycin B, Streptomyces sp., Sterile-Filtered Solution in PBS, Cell Culture-Tested - CAS 31282-04-9 - Calbiochem. Hygromycin B is an aminoglycoside antibiotic …

WebThus, it directly affects the tea industry qualitatively and quantitatively. Solanum tuberosum class I chitinase gene (AF153195) is a plant pathogenesis-related gene. It was … Webbinary vector carrying the neomycin phosphotransferase II (nptII), hygromycin phosphotransferase (hpt), and intron-containing β-glucuronidase (gus-intron) genes in …

Webmarker-off. The hygromycin phosphotransferase gene (hpt) from E. coli which has been proved to be very effective marker for plant transformation was used in this study. The … Web在大肠杆菌中进行了HPT蛋白质的重组表达,利用纯化的HPT蛋白质制备了其特异性的单克隆抗体,采用免疫印迹学方法对转基因水稻4021中HPT蛋白质的表达情况进行了检测,该方 …

WebThe gene codes for hygromycin phosphotransferase (HPT), which detoxifies the aminocyclitol antibiotic hygromycin B. A large number of plants have been transformed …

Web嘌呤霉素 (Puromycin) 来源于白黑链霉菌 (Streptomyces alboniger)代谢产物的一种氨基糖苷类抗生素，能抑制原生生物、藻类、哺乳动物和昆虫细胞的生长, 一般2天内能杀 … loftridge hoaWebAbstract. Embryogenic maize ( Zea mays L.) callus cultures were transformed by microprojectile bombardment with a chimeric hygromycin phosphotransferase (HPT) … loft ridge beamWebHPT, the hygromycin phosphotransferase gene. (B) Target sites for eight sgRNAs targeting HD1. The gene structure (upper) and partial abbreviate sequence (lower) of … ind pubgWeb5 jun. 2008 · Hundreds of hygromycin-resistant plants per sporangium were obtained by co-cultivation of immature thalli with Agrobacterium carrying the binary vector that … ind puglieseWeb2 sep. 2024 · The hygromycin phosphotransferase (HPT) gene as a selective marker is normally used in screening tests as a first step in detecting and quantifying genetically … indpulse wearWebEmbryogenic calli induced after 20 days of highly regenerating rice cultivar BRRI dhan29 and Agrobacterium strain GV 3101 was transformed with binary vector pCAMBIA 1303 … loft ridge homeowners association indpulse wear avis