WebShanghai Biowing Applied Biotechnology Co., Ltd. (Shanghai, China) via STR profiling. All cells were cultured in a humidified incubator at 37 °C with 5% CO 2. Cell lines were cultured in RPMI-1640 medium ... Institute of Biotechnology), after which, signals were visualized using a UCECL Plus kit (Amersham, Buckinghamshire, UK). At least 3 ... WebThe absence of contamination of the three cell lines was confirmed by short tandem repeat analysis (Biowing applied biotechnology Co., Ltd, Shanghai, China). ... A lysis buffer was applied to extract the total protein of cells, then a BCA assay kit was used to evaluate the protein concentrations. All kits were provided by Beyotime Biotechnology ...
Cancer-associated fibroblast-derived SDF-1 induces epithelial ...
WebShort tandem repeat authentication was conducted by Shanghai Biowing Applied Biotechnology Co., Ltd. (Shanghai, China). MEC-1 was established from the peripheral blood of a 61-year-old Caucasian man with chronic B cell leukemia. JVM-3 was constructed from the peripheral blood of a 73-year-old man with B-prolymphocytic leukemia. WebDec 15, 2024 · Blood samples were collected from all participants using K2EDTA tubes and a Blood Genotyping DNA Extraction Kit. The genotype of the SNP was analyzed by the Shanghai Biowing Applied Biotechnology Co. Ltd. (www.biowing.com.cn) using the Ligase Detection Reaction-Polymerase Chain Reaction method. Genomic DNA extracted … imvelo office furniture
Establishment of an induced pluripotent stem cell line (JTUi003-A) …
WebSNPs test by the Shanghai Biowing Applied Biotechnology company. Specic primers were designed for the sites to be detected, and the target region was re-sequenced by … WebApr 7, 2024 · Population 2 contains 303 individuals from multiple families with similar day age. The top 50 candidate SNPs with the smallest p values obtained in GWAS were genotyped in population 2 by the target sequencing method (Shanghai Biowing Applied Biotechnology Co., Ltd., Shanghai, China). The general linear model (GLM) was used … WebFeb 5, 2008 · Hypericin, extracted from H. perforatum, can induce the generation of reactive oxygen species by visible light irradiation, which may consequently induce the conformational change of hemoglobin. We have not only employed UV-vis spectroscopy to observe the changes of UV-vis spectra of the protein, which reveals the conformational … imvelo wealth solutions